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Recombinant Mouse TGF-beta 1 Protein

(7666-MB-005)



概述
货号 7666-MB-005
别名 CEDLAP; DPD1; latency-associated peptide; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1
全称 Transforming Growth Factor beta 1
反应种属 Mouse
形式 Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
性能
供应商 R&D Systems
Entrez Gene IDs 7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts
Authors: Koji Ueno
Sci Rep, 2016;6(0):28538.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
T cells are necessary for ILC2 activation in house dust mite-induced allergic airway inflammation in mice
Authors: BW Li, MJ de Bruijn, I Tindemans, M Lukkes, A KleinJan, HC Hoogsteden, RW Hendriks
Eur J Immunol, 2016;0(0):.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Affinity for self antigen selects Treg cells with distinct functional properties
Nat Immunol, 2016;0(0):.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Autonomous membrane IgE signaling prevents IgE-memory formation
Nat Immunol, 2016;0(0):.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Vimentin coordinates fibroblast proliferation and keratinocyte differentiation in wound healing via TGF-?-Slug signaling
Proc Natl Acad Sci USA, 2016;113(30):E4320-7.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Reduced TCR-dependent activation through citrullination of a T-cell epitope enhances Th17 development by disruption of the STAT3/5 balance
Eur J Immunol, 2016;0(0):.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Leptin directly promotes T-cell glycolytic metabolism to drive effector T-cell differentiation in a mouse model of autoimmunity
Eur J Immunol, 2016;0(0):.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells
Authors: I Meininger, RA Griesbach, D Hu, T Gehring, T Seeholzer, A Bertossi, J Kranich, A Oeckinghau, AC Eitelhuber, U Greczmiel, A Gewies, M Schmidt-Su, J Ruland, T Brocker, V Heissmeyer, F Heyd, D Krappmann
Nat Commun, 2016;7(0):11292.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Regulatory T cell reprogramming toward a Th2-cell-like lineage impairs oral tolerance and promotes food allergy.
Authors: Noval Rivas M, Burton O, Wise P, Charbonnier L, Georgiev P, Oettgen H, Rachid R, Chatila T
Immunity, 2015;42(3):512-23.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Myocardin-related transcription factors control the motility of epicardium-derived cells and the maturation of coronary vessels.
Authors: Trembley M, Velasquez L, de Mesy Bentley K, Small E
Development, 2015;142(1):21-30.

Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1.
Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
Proc Natl Acad Sci U S A, 2015;112(9):E957-65.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
ShcA regulates late stages of T cell development and peripheral CD4+ T cell numbers.
Authors: Buckley M, Trampont P, Arandjelovic S, Fond A, Juncadella I, Ravichandran K
J Immunol, 2015;194(4):1665-76.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
A functional polymorphism of Ptpn22 is associated with type 1 diabetes in the BioBreeding rat.
Authors: Sarmiento J, Wallis R, Ning T, Marandi L, Chao G, Veillette A, Lernmark A, Paterson A, Poussier P
J Immunol, 2015;194(2):615-29.
Species: Rat
Sample Type: Whole Cells
Application: Bioassay
Novel mechanism of macrophage-mediated metastasis revealed in a zebrafish model of tumor development.
Authors: Wang J, Cao Z, Zhang X, Nakamura M, Sun M, Hartman J, Harris R, Sun Y, Cao Y
Cancer Res, 2015;75(2):306-15.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Beclin 1 regulates neuronal transforming growth factor-beta signaling by mediating recycling of the type I receptor ALK5.
Authors: O'Brien C, Bonanno L, Zhang H, Wyss-Coray T
Mol Neurodegener, 2015;10(1):69.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Itk signals promote neuroinflammation by regulating CD4+ T-cell activation and trafficking.
Authors: Kannan A, Kim D, August A, Bynoe M
J Neurosci, 2015;35(1):221-33.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
IL-1 signaling modulates activation of STAT transcription factors to antagonize retinoic acid signaling and control the TH17 cell-iTreg cell balance.
Authors: Basu R, Whitley S, Bhaumik S, Zindl C, Schoeb T, Benveniste E, Pear W, Hatton R, Weaver C
Nat Immunol, 2015;16(3):286-95.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Cell-extrinsic CTLA4-mediated regulation of dendritic cell maturation depends on STAT3.
Authors: Kowalczyk A, D'Souza C, Zhang L
Eur J Immunol, 2014;44(4):1143-55.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
IL-21 induces IL-22 production in CD4+ T cells.
Authors: Yeste A, Mascanfroni I, Nadeau M, Burns E, Tukpah A, Santiago A, Wu C, Patel B, Kumar D, Quintana F
Nat Commun, 2014;5(0):3753.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Myostatin stimulates, not inihibits, C2C12 myoblast proliferation.
Authors: Rodgers B, Wiedeback B, Hoversten K, Jackson M, Walker R, Thompson T
Endocrinology, 2014;155(3):670-5.
Species: Mouse
Sample Type: whole cells
Application: Bioassay
Retinoic acid prevents mesenteric lymph node dendritic cells from inducing IL-13-producing inflammatory Th2 cells.
Authors: Yokota-Nakatsuma A, Takeuchi H, Ohoka Y, Kato C, Song S, Hoshino T, Yagita H, Ohteki T, Iwata M
Mucosal Immunol, 2014;7(4):786-801.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Laminin alpha1 regulates age-related mesangial cell proliferation and mesangial matrix accumulation through the TGF-beta pathway.
Authors: Ning L, Kurihara H, de Vega S, Ichikawa-Tomikawa N, Xu Z, Nonaka R, Kazuno S, Yamada Y, Miner J, Arikawa-Hirasawa E
Am J Pathol, 2014;184(6):1683-94.
Species: Mouse
Sample Type: N/A
Application: ELISA development
Attenuation of experimental colitis in glutathione peroxidase 1 and catalase double knockout mice through enhancing regulatory T cell function.
Authors: Kim H, Lee A, Choi E, Kie J, Lim W, Lee H, Moon B, Seoh J
PLoS ONE, 2014;9(4):e95332.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
FAK-related nonkinase is a multifunctional negative regulator of pulmonary fibrosis.
Authors: Ding Q, Cai G, Hu M, Yang Y, Zheng A, Tang Q, Gladson C, Hayasaka H, Wu H, You Z, Southern B, Grove L, Rahaman S, Fang H, Olman M
Am J Pathol, 2013;182(5):1572-84.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Endothelial dysfunction exacerbates renal interstitial fibrosis through enhancing fibroblast Smad3 linker phosphorylation in the mouse obstructed kidney.
Authors: Sun Y, Qu X, Li X, Nikolic-Paterson D, Li J
PLoS ONE, 2013;8(12):e84063.
Species: Rat
Sample Type: whole cells
Application: cell culture
Exposure to atmospheric particulate matter enhances Th17 polarization through the aryl hydrocarbon receptor.
Authors: van Voorhis, Michael, Knopp, Samantha, Julliard, Walker, Fechner, John H, Zhang, Xiaoji, Schauer, James J, Mezrich, Joshua D
PLoS ONE, 2013;8(12):e82545.
Species: Mouse
Sample Type: whole cells
Application: cell culture
Regulation of innate CD8+ T-cell activation mediated by cytokines.
Proc Natl Acad Sci U S A, 2012;109(25):9971-6.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay

内毒素水平 <0.01 EU per 1 μg of the protein by the LAL method.
生物活性 Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is typically 0.04-0.2 ng/mL.
溶解方法 Reconstitute at 50 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
背景

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF‑ beta superfamily that share a characteristic cystine knot structure (1‑7). TGF‑ beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial‑mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non‑redundant functions; for TGF‑ beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human or mouse TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature mouse TGF‑ beta 1 shares 99‑100% aa sequence identity with human, rat, equine, porcine, canine and bovine TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta  RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta  RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta  RIII) and endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

Accession # P04202
运输条件 Blue Ice
N-terminal
Sequence Analysis
Ala279
预期分子量 12.8 (monomer) kDa
SDS-PAGE 10.5 kDa, reducing conditions
存放说明 M-20C
纯度 >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
不含载体 What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

参考文献
  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
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¥4821.9

货号:7666-MB-005

规格: 5ug
数量:
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